System overview
Abstract
Single-shot volumetric fluorescence (SVF) imaging captures biological processes with high temporal resolution and a large field of view, unlike traditional methods requiring multiple axial plane scans. Existing SVF methods often face limitations due to large, complex point spread functions (PSFs), affecting signal-to-noise ratio, resolution, and field of view. The paper introduces the QuadraPol PSF combined with neural fields, using a compact custom polarizer and a polarization camera to detect fluorescence and encode the 3D scene within a compact PSF without depth ambiguity. This approach, coupled with a novel reconstruction algorithm, significantly reduces acquisition time by approximately 20 times and captures a 100 mm³ volume in one shot, demonstrated through imaging bacterial colonies and plant root morphology.
Bacteria Colony on Sand Surfaces
All-in-focus image
Focal stack
Plant root volumetric imaging
Image volume with color-coded depth
Zoom-in slices
Comparison of neural fields and deconvolution for zoom-in regions (xy, xz planes) of the plant root. Depth infomation is encoded in colors.
Focal stack for plant root
BibTeX
@article{zhang2025single,
title={Single-shot volumetric fluorescence imaging with neural fields},
author={Zhang, Oumeng and Zhou, Haowen and Feng, Brandon Y and Larsson, Elin M and Alcalde, Reinaldo E and Yin, Siyuan and Deng, Catherine and Yang, Changhuei},
journal={Advanced Photonics},
volume={7},
number={2},
pages={026001--026001},
year={2025},
publisher={Society of Photo-Optical Instrumentation Engineers}
}